Part:BBa_K4825016
ADH7
ADH7 is derived from S.cerevisiae genome encoding for an alcohol dehydrogenase (ADH), which catalyzes the conversion of aldehydes to the corresponding alcohols. In the pathway of producing isopentanol, the termination enzymes, ketoacid decarboxylases (KDCs) and alcohol dehydrogenases (ADHs), convert n-carbon ketoacids to their corresponding (n − 1)-carbon alcohols. The part collection includes ARO10 (2-ketoacid dehydrogenase), ADH7 and ATF1 (alcohol acetyltransferase). ARO10 and ADH7 catalyze the reaction to convert 2-ketoacid to isopentanol, and ATF1 further catalyze the reaction from isopentanol to isopentyl acetate (also named isoamyl acetate). The part collection can offer an efficient pathway and inspire the future team to design the pathway of producing isoamyl acetate efficiently, and obtaining the banana-like odor.
Usage and Biology
ADH7 is derived from the genome of S.cerevisiae encoding for an alcohol dehydrogenase (ADH). During the fermentation of yeast, it catalyzes the production of n-carbon alcohols by converting n-carbon ketoacids to their corresponding (n − 1)-carbon alcohols along with KDCs (ketoacid decarboxylases) and terminates the recursive pathway which catalyzes the elongation of a 2-ketoacid by one carbon atom.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 471
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 232
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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